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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Nanomedicine. 2011 Nov 22;8(6):1017–1025. doi: 10.1016/j.nano.2011.11.009

Table 1.

ASOs used for Detection of PCA3 RNA transcripts.

Name Sequence (5’ to 3’) Description
ASO468 TGACCCAAGATGGCGGCCGG ASO targeting PCA3 at the region immediately 3’ to nt 468
ASO683 TGCCATCAAGATTTTCTCGTC ASO targeting PCA3 at the region immediately 3’ to nt 683
ASO735 GCT GCC TCA TGT CAT CAC AG ASO targeting PCA3 at the region immediately 3’ to nt 735
ASO735 MM1 GCT GCC TCA TCT CAT CAC AG Single-nt mismatch of ASO735
ASO735 MM2A GCT GCC TCA TCT CAT CAC GG Two-nt mismatch of ASO735
ASO735 MM2B GCT GCC TCA TCG CAT CAC AG Two-nt mismatch of ASO735
ASO735 MM4 GCT GCC TCA TCG AGT CAC AG Four-nt mismatch of ASO735
DNA mimic of PCA3 CTG TGA TGA CAT GAG GCA GCT TTT TGA CGA GAA AAT CTT GAT GGC 45-nt sequence containing the two portions of PCA3 that the ASOs were complementary to, separated by 5 T's
ASOT683 (TTT) TTT TTT TTG CCA TCA AGA TTT TCT CGTC ASO683 with 7 or 10 Ts added at the 5’ end as noted
ASOT735 (TTT) TTT TTT TGC TGC CTC ATG TCA TCA CAG ASO735 with 7 or 10 Ts added at the 5’ end as noted
ASOnon CTC GTA TCT CAA CTC GTA TTT TTT TTT TTT Non-complementary probe sequence