Fig. 5.

Determination of promoter activity by reporter assay. a Schematic representation of different promoter reporter constructs used for promoter mapping of the ORF30-33 locus in KSHV genome. Positions for the cloned promoters are also shown and labeled as P1–4, respectively. b Basal activity of promoters from the ORF30-33 locus. The relative activity of vector control pGL4 is shown as “1”. c Deletion of TATA boxes from the promoters in the ORF30-33 locus reduced promoter activities. d RTA failed to activate the promoter reporters from the ORF30-33 locus. vIL6 promoter, which is highly responsive to RTA, was used as a positive control in the reporter assay. Results were presented as mean and error bars from three independent experiments, each with triplicates