Figure 6. C1899/1903G disulfide mutation confers secretion advantage in vivo in a hemophilia A mouse model.
FVIII antigen (Panel A) and activity (Panel B) in the plasma of hemophilia A mice were measured by an ELISA and a COAMATIC chromogenic assay respectively, following transient expression of 226/N6-F309S and 226/N6-DM-F309S constructs by hydrodynamic tail vein injection as described under Methods. Data presented (○) represent multiple independent expression studies (N ≥ 5) with the mean indicated by a bar. *P < 0.05