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. Author manuscript; available in PMC: 2013 Feb 9.
Published in final edited form as: Oncogene. 2011 Nov 28;31(32):3721–3732. doi: 10.1038/onc.2011.534

Figure 6. ARF stimulates MDMX ubiquitination and degradation by MDM2.

Figure 6

(a) MDMX was co-transfected with MDM2, ARF and His6-ubiquitin into H1299 cells. Ubiquitinated MDMX was detected by Ni-NTA pull down followed by MDMX Western blot. (b) MDMX-367A was co-transfected with MDM2, ARF and His6-ubiquitin into U2OS cells. Ubiquitinated MDMX-367A was detected by Ni-NTA pull down followed by MDMX Western blot. Note the increase of poly ubiquitinated MDMX in the presence of ARF (upper bracket). (c) MDMX-367A was co-transfected with MDM2 and ARF into U2OS cells. MDMX-367A degradation was analyzed by Western blot.