Skip to main content
. Author manuscript; available in PMC: 2013 Feb 9.
Published in final edited form as: Oncogene. 2011 Nov 28;31(32):3721–3732. doi: 10.1038/onc.2011.534

Figure 8. ARF stimulates MDMX-MDM2 binding.

Figure 8

(a) MDMX was co-transfected with MDM2 and ARF in U2OS cells. Co-precipitation of MDM2 and MDMX was analyzed by MDM2 IP followed by MDMX Western blot. (b) NARF6 cells were treated with IPTG (to induce ARF) or Nutlin (to induce comparable level of MDM2) for 16 hrs and analyzed for endogenous MDMX-MDM2 binding by IP-Western blot. (c) MDMX was co-transfected with MDM2 and ARF into U2OS cells. MG132 was added 4 hrs before the cells were harvested. Co-precipitation of MDM2 and MDMX was analyzed by MDM2 IP followed by MDMX Western blot.