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. Author manuscript; available in PMC: 2013 Feb 16.
Published in final edited form as: Oncogene. 2011 Nov 28;31(33):3785–3795. doi: 10.1038/onc.2011.536

Figure 5.

Figure 5

MYCN activates CCNE1 transcription. a, qRT-PCR analysis of cyclin mRNA levels in SHEP1 cells infected with retroviruses expressing either GFP or MYCN (error bars, s.d., n=3). Two-tailed Student's t-test analysis revealed only significant upregulation of CCNE1 mRNA. b, Immunoblot analysis of cyclin E1 and MYCN levels in SHEP1 cells expressing either GFP or MYCN. Levels of cyclin E1 were quantified against β-actin with the cyclin E1 level in GFP-expressing SHEP1 cells being designated as 1.0. c, Scatter dot plots of CCNE1 expression levels in primary neuroblastomas with or without MYCN amplification. The data were analyzed using two-tailed Student's t-test with p values indicated.