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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Mol Cancer Res. 2011 Jul 1;9(8):1067–1077. doi: 10.1158/1541-7786.MCR-10-0445

Figure 2. Androgens upregulate Drp1 mRNA and protein expression.

Figure 2

A. In the left panel, cells were grown in medium containing 9% FBS or charcoal/dextran-stripped serum (CSS); and in the right panel, cells were treated with R1881 (10nM) for 24h. Total cell lysates were analyzed by western blot for the levels of Drp1, PSA and AR. Hsp90α was used as a loading control. B. Total RNA was isolated and Drp1 (left) and PSA (right) mRNA were measured by qRT-PCR (**P≤0.01; ***P≤0.001, N=3). C. Cells were treated with increasing concentrations of R1881 for 24h and total cell lysates were analyzed for the levels of Drp1 protein. D. Cells were treated with R1881 for different periods and Drp1 mRNA expression was analyzed by qRT-PCR (P values were compared to the CSS controls, N=3).