Table 1.
Mobile phase condition of chromatographic separation (254 nm).
| Time (min) |
Water (in 2% acetic acid) |
Acetonitrile (in 2% acetic acid) |
Flow rate (mL/min) |
|---|---|---|---|
| 0 | 95 | 5 | 1 |
| 5 | 95 | 5 | 1 |
| 60 | 20 | 80 | 1 |
| 70 | 20 | 80 | 1 |
| 75 | 95 | 5 | 1 |
| 90 | 95 | 5 | 1 |
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