Figure 2.
Phylogenetic tree of concatenated sequences of fragments of the glnA gene (474 bp) and the gyrB gene (576 bp) in 52 Photorhabdus isolates representing known diversity across the genus. The tree was constructed with the neighbor-joining algorithm and the K2-P method of distance estimation as implemented in MEGA version 3.0 (12). A total of 1,000 bootstrap replicates were performed, and the percentage of bootstrap trees supporting each node are given. The Kingscliff isolate (arrow) clusters with P. asymbiotica isolates from Australia, both in the concatenated tree (bootstrap score = 100%) and in individual gene trees (not shown). The scale bar shows percentage relatedness.