Figure 3.

The number of mast cells and eosinophils of the dorsal skin with histopathological analysis. AD induced in Nc/Nga mice by topical application of 2,4-dinitrochlorobenzene (DNCB) in the dorsal skin, and a right ear was topically treated with 1,3-butylen glycol (BG; control), 5% Chrysanthemum indicum L. (CIL-Low), 30% Chrysanthemum indicum L. (CIL-High), and 0.1% hydrocortisone (HC) on the lesions for 5 weeks. Normal controls did not have DCNB-induced AD. After 5 weeks of treatments, the dorsal skin was fixed with 10% formaldehyde, embedded in paraffin, and then sections were made. The skin sections were stained with hematoxylin and eosin and toluidine blue staining. CIL-High significantly elevated mast cells and eosinophils in the lesion of the dorsal skin in comparison to the control and exhibited a statistically similar increment to HC. Each value represents the mean ± SD of 5 mice in each group. (a) The number of eosinophils as determined by hematoxylin and eosin staining. Eosinophils exhibited as blue dots. (b) The number of mast cells determined by toluidine blue staining. Mast cells appear as blue dots. *Significantly different among the different treatments in Nc/Nga mice at P < 0.05. a, b, c Means on the bars with different superscripts were significantly different by Tukey test at P < 0.05. ^†Significantly different between control (BG) and normal control at P < 0.05.