Figure 3.

Effects of 143B and 143Bρ ⁰ cells with P. urinaria-induced intracellular and mitochondrial ROS generation and mitochondrial membrane potential. The intracellular ROS with DCFDA staining by FACS analysis in 143B and 143Bρ ⁰ cells for 24 (a) and 48 (b) hours. The values are displayed as the mean ± SD of the mean fluorescence intensity of DCF. 143B and 143Bρ ⁰ cells were preincubated with MitoSOX Red and DAPI in the absence or presence of P. urinaria 2 mg/mL treatment for 24 (c) and 48 (d) hours. Representative digital images of MitoSOX and DAPI fluorescence were obtained using a fluorescence microscope (1000x). The mitochondrial membrane potential (ΔΨm) stained with Rhodamine 123 by FACS analysis in 143B and 143Bρ ⁰ cells for 24 (e) and 48 (f) hours. The values are displayed as the mean ± SD of percent control on intracellular ROS and mitochondrial membrane potential. Three independent experiments in duplicate were performed. Asterisks represent statistical significance from the vehicle control (*P < 0.05, **P < 0.01). A pound sign represents statistical significance between 143B and 143Bρ ⁰ cells (^# P < 0.05).