Figure 5.

Effect of catenarin on MAPK phosphorylation elicited by MIP-1β or SDF-1β. One million JK-EF1α-CCR5 cells (a) and Jurkat cells (b) were pretreated with vehicle or catenarin (1 μg/mL) for 0, 5, 10, and 15 min, followed by the addition of (a) MIP-1β (100 ng/mL) or (b) SDF-1β (100 ng/mL). Total lysates were immunoblotted with the Abs against MAPKs (p38, JNK, and ERK) or their phosphorylated proteins. Representative data and the ratio of the signal of the phosphorylated MAPKs to that of total MAPKs are shown.