Table 2.
Library | Probe | Number of plaques screened (× 103) | Number of sequences obtained | Number of real olfactory receptor sequences | True-positive rate | Olfactory receptor clone frequency | Number of olfactory receptor genes represented |
Embryonic | OR5B_OR3B_40 | 640 | 58 | 37 | 64% | 1/17,300 | 27 |
Adult | OR5B_OR3B_40 | 2,850 | 1,450 | 1,138 | 78% | 1/2,500 | 394 |
P24_P28_40 and TM3deg1_P28_45 | 200 | 23 | 3 | 13% | 1/66,700 | 3 | |
P26_P27_45 | 700 | 135 | 58 | 43% | 1/12,100 | 35 | |
P24_P28_45 | 200 | 39 | 22 | 56% | 1/9,100 | 19 | |
OR5B_OR3B_45 | 150 | 10 | 6 | 60% | 1/250,000 | 5 | |
Total | 4,740 | 1,715 | 1,264 | 74% | 1/3,800 | 419 |
Probe names comprise the names of the two primers and the annealing temperature used during PCR to generate the probes, separated by underscores