Table IV.

A Basic Workflow of an Automated DOE for Optimizing a LBA

DOE design (factors, levels, number of conditions, etc.) is used to generate a plate format
The user loads the automated liquid handler with the necessary labware, reagents, and buffers.
The automated liquid handler performs the complex dilutions and additions of the reagents in intermediate plates according to the plate format.
A multichannel pipettor (integrated or stand-alone) transfers the diluted reagents to the assay plate.