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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Biochem J. 2011 Apr 1;435(1):175–185. doi: 10.1042/BJ20100840

Figure 5. Apoptosis in UHRF1 depleted cells occurs through the activation of caspase-8.

Figure 5

A) Proteolytic fragments of caspase-8 representing its activated form (p43/p41 and p18) are detected in UHRF1 depleted cells but not in UHRF1 containing cells (panel 2, compare lanes 2 and 3 with 1). B). Immunofluoresence photographs (right panel, green) of UHRF1 containing and depleted cells show that activated caspase-8 is present in cells lacking UHRF1. Phase photographs are also shown for comparison (left) C) Depletion of total caspase-8 prevents PARP and caspase-3 cleavage. p89 PARP and activated caspase-3 levels increase in UHRF1 depleted but caspase-8 containing cells (panels 3 and 4, lane 2) while low in caspase-8 depleted (C8A) but UHRF1 containing cells (panels 3 and 4, lane 3). When caspase-8 in depleted in cells lacking UHRF1, PARP and caspase-3 cleavage are suppressed (panels 3 and 4, lane 4). Note that “Caspase 8” represents total caspase-8 levels while “Caspase 8 (activated)” represents proteolyzed forms of caspase-8.