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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Biochem J. 2011 Apr 1;435(1):175–185. doi: 10.1042/BJ20100840

Figure 7.

Figure 7

UHRF1 localizes to UV laser scissor induced DNA damage. A) phospho- H2AX accumulates at site of DNA damage after targeted injury (first row, green). UHRF1 is recruited to these sites within 5 minutes (second row, red), peaks in intensity at 30 minutes and then begins to fade. B) Quantitation of UHRF1 localization with pH2AX. The ratio of intensity of UHRF1 at region of laser scissors induced damage (marked by pH2AX) compared to undamaged region is plotted. Note that the change in intensity of UHRF1 staining is dynamic and differs from that of phospho-H2AX. C) Flag-UHRF1 localizes to DNA damage. Cells were transfected with Flag-UHRF1 (top panel) or empty vector (bottom panel). Cells were subjected to UV laser and processed for immunofluorescence 30 min post DNA damage with pH2AX and Flag antibodies. UHRF1 is present in the stripe in transfected cells (top, column 2) but not in control cells (bottom, column 2).