Figure 7.

Effects of point mutations in positions 207 and 238 of the TsHKT1;2 protein. Yeast strain CY162 (Mat a, ura3-52, his3D200, his44-15, trkD1, trkD2::pcK64) cells harboring TsHKT1;2 were compared with cells expressing point mutants TsHKT1;2-1 (D207N), TsHKT1;2-2 (D238N), and TsHKT1;2-1/2 (D207N and D238N). Cells were spotted in decimal dilution on AP medium containing either 100 mm KCl (Control) or the indicated amounts of KCl and NaCl. Cells transformed with the vector control, AtHKT1, and AtKAT1 are included as controls. [See online article for color version of this figure.]