Table I. Intracellular ROS levels in P. ginseng and Arabidopsis control and rolB-expressing cell cultures measured by laser confocal microscopy.
P. ginseng and Arabidopsis calli or cell suspensions were cultivated for 21 or 5 d, respectively, and analyzed by confocal microscopy. ROS levels are presented as mean ± se from a single experiment. *P < 0.05 versus values of the corresponding controls, Student's t test.
| Cell Line | No. of Cells Analyzed | DCF Fluorescence, Pixels |
| P. ginseng calli (vector control) | 58 | 65.4 ± 3.9 |
| P. ginseng rolB-expressing calli | 79 | 48.2 ± 2.4* |
| P. ginseng cell suspension (vector control) | 80 | 60.0 ± 4.5 |
| P. ginseng rolB-expressing cell suspension | 80 | 48.5 ± 2.0* |
| Arabidopsis calli (vector control) | 100 | 75 ± 3.5 |
| Arabidopsis low-rolB-expressing calli AtB-1 | 100 | 60 ± 2.5* |
| Arabidopsis high-rolB-expressing calli AtB-2 | 100 | 47.5 ± 1.9* |
| Arabidopsis cell suspension (vector control) | 80 | 86.1 ± 5.0 |
| Arabidopsis low-rolB-expressing cell suspension AtB-1 | 80 | 66.4 ± 4.5* |
| Arabidopsis high-rolB-expressing cell suspension AtB-2 | 80 | 56.5 ± 4.2* |