Table II.
Pairwise comparison of spikelet fertility between transgenic plants expressing the RNAi vector and negative controls
| Generation | Receptora | Average Fertility |
Fertility Difference | Pd | |
| Transgenicb | Controlc | ||||
| % | |||||
| T0 | S5jS5j-Balilla (1) | 86.64 | 87.93 | −1.29 | 0.5940 |
| S5jS5j-Balilla (2) | 92.99 | 96.66 | −3.67 | 0.3458 | |
| S5iS5i-Balilla (1) | 86.70 | 86.71 | −0.01 | 0.9993 | |
| S5iS5i-Balilla (2) | 93.51 | 86.98 | 6.52 | 0.2406 | |
| S5nS5n-Balilla | 91.19 | 95.04 | −3.85 | 0.7189 | |
| T1 | S5jS5j-Balilla | 80.10 | 81.92 | −1.81 | 0.4471 |
| S5iS5i-Balilla | 87.19 | 87.97 | −0.78 | 0.4813 | |
| S5nS5n-Balilla | 81.80 | 88.77 | −6.98 | 0.3380 | |
| Wuxiangjing | 88.11 | 89.26 | −1.15 | 0.4599 | |
a
(1) and (2) denote two environmental conditions in which spikelet fertility was tested.
b
Transgenic plants (i.e. transformed with the RNAi vector).
c
Control plants that did not contain the RNAi vector.
d
Significant difference in spikelet fertility between transgenic and control plants (P < 0.05). In the T0 generation, plants transformed with a blank vector served as the negative control, whereas in the T1 generation, nontransgenic plants were used as the negative control.