Skip to main content
. 2011 Dec 30;158(3):1426–1438. doi: 10.1104/pp.111.192385

Figure 6.

Figure 6.

GFP:VLN3, expressed under the control of the VLN3 promoter, localizes to (bundles of) actin filaments. A to D, Representative images of complemented vln2 vln3 plants show that besides a cytoplasmic localization, GFP:VLN3 decorates (bundles of) actin filaments in hypocotyl epidermal (A), leaf epidermal (B), and root epidermal (C and D) cells. These images show fully grown hypocotyl epidermal (A) and leaf epidermal (B) cells and elongating root epidermal cells (C and D). In growing root hairs (D), GFP:VLN3 localizes to the long actin filament bundles oriented longitudinally to the cell’s long axis in the root hair tube. E to G, Coexpression of PVLN3:GFP:VLN3 and P35S:mCherry:FABD2 in tobacco demonstrates that GFP:VLN3 (E) and mCherry:FABD2 (F) colocalize (arrows) in leaf epidermal cells, although GFP:VLN3 does not localize to all actin filaments (arrowheads). G shows an overlay of E and F (GFP:VLN3, green; mCerry:FABD2, magenta). Image sequences of elongating root epidermal cells (H–J and K–M; root hairs) of complemented vln2 vln3 plants show that GFP:VLN3 localizes to (bundles of) actin filaments that reorganize over time. See also Supplemental Movies S2 and S3. Bars = 10 μm. [See online article for color version of this figure.]