Table 1. Conditions for Aspergillus ustus DNA amplification.

	Primers
	Ustus 1	R151	RPO2	OPA10	OPA20
Primer sequence	5´-GTA TTG CCC T-3´	5´-GCT GTA GTG T-3´	5´-GCG ATC CCC A-3´	5´-GTG ATC GCA G-3´	5´-GTT GCG ATC C-3´
Primer concentration	0.8 pmol/L	1.0 pmol/L	1.0 pmol/L	0.4 pmol/L	1.0 pmol/L
MgCl2	1.8 mmol/L	2.2 mmol/L	3.0 mmol/L	1.8 mmol/L	2.0 mmol/L
Template concentration	0.025 ng/50μL	0.5 ng/50μL	0.012 ng/50μL	0.03 ng/50μL	0.1 ng/50μL
Annealing temperature	32°C	32°C	34°C	32°C	32°C
Annealing time	1.5 min	1.5 min	1.5 min	1.5 min	1.5 min