Table 1. Conditions for Aspergillus ustus DNA amplification.
Primers |
|||||
---|---|---|---|---|---|
Ustus 1 | R151 | RPO2 | OPA10 | OPA20 | |
Primer sequence | 5´-GTA TTG CCC T-3´ | 5´-GCT GTA GTG T-3´ | 5´-GCG ATC CCC A-3´ | 5´-GTG ATC GCA G-3´ | 5´-GTT GCG ATC C-3´ |
Primer concentration | 0.8 pmol/L | 1.0 pmol/L | 1.0 pmol/L | 0.4 pmol/L | 1.0 pmol/L |
MgCl2 | 1.8 mmol/L | 2.2 mmol/L | 3.0 mmol/L | 1.8 mmol/L | 2.0 mmol/L |
Template concentration | 0.025 ng/50μL | 0.5 ng/50μL | 0.012 ng/50μL | 0.03 ng/50μL | 0.1 ng/50μL |
Annealing temperature | 32°C | 32°C | 34°C | 32°C | 32°C |
Annealing time | 1.5 min | 1.5 min | 1.5 min | 1.5 min | 1.5 min |