Table 1 . Deficiency screen for novel regulators in GSC self-renewal.

Function Groups	Gene Name	Allele(s)	Function	% Clonal Day14a	GSC Loss/Dayb	Passed 2′ Screen
I. Ubiquitin ligase	POSH	hsFLP; FRT42DPOSHk15815/FRT42DGFP	E3 ubiquitin ligase	0%	30%	Yes
	Pie	hsFLP; pieE1-16FRT40A/FRT40AGFP	E3 ubiquitin ligase (Predicted)	0%	28.76%	Yes
		hsFLP; pief05500 FRT40A/FRT40AGFP		0%	25%
	UbcD10	hsFLP; FRT42DUbcD10BG00902/FRT42DGFP	E2 ubiquitin ligase	3.40%	/	Yes
II. EGFR signaling	Kekkon-1	hsFLP; kek1k07332 FRT40A/FRT40AGFP	Repressor of EGFR signaling	11%	9%	Yes
	drk	hsFLP; FRT42Ddrkk02401/FRT42DGFP	RTK adaptor protein	4.80%	7.59%	Yes
		hsFLP; FRT42BdrkΔP24/FRT42BGFP		25.00%	4.08%
III. Transcription factors	Abrupt	hsFLP; abk02807 FRT40A/ FRT40AGFP	Transcription factor activity Specific RNA polymerase II	6.9%	10.5%	Yes
	Cropped	hsFLP; crpKG08234FRT40A/FRT40AGFP	Transcription factor	0%	28.7%	Yes
IV. Housekeeping genes	CG8674	hsFLP; k14505k14505FRT40A/FRT40AGFP	Proton-transporting ATP synthase complex assembly	0%	/	Yes
	Df31	hsFLP; Df31k05815FRT40A/FRT40AGFP	chromatin organization	0%	/	Yes
	snRNA:U6atac:29B	hsFLP;snRNA:U6atac:29Bk01105FRT40A/ FRT40AGFP	nuclear mRNA splicing	6%	/	Yes

EGFR, epidermal growth factor receptor; GSC, germline stem cell; RTK, receptor tyrosine kinase.

^a&.

^bn > 80.