Figure 3 .

Changes in MZ-CZ organization in mutant lymph glands. Confocal projections of late third-instar lymph glands of wild type (A), vsg^EY05552 (B) homozygous mutants, and l(2)gl⁴ Df(2L)net62 (C). The relative size of the CZ (anti-Pxn, membrane-cytoplasmic, red) in both mutants is increased, whereas the MZ (Pxn-negative) size is decreased. Severe reduction of the MZ is also seen in early middle third-instar nopo^null lymph glands (G, G′) compared with wild-type lymph glands at the same stage (D). In Cdc27^L7123 and mus304^D3 mutants (E, F) the relative sizes of MZ and CZ are not significantly changed. Staining with phalloidin (green) shows the burst of lamellocytes in cross-sections through the inner lymph gland layers (F′, G′) in one lobe of mus304^D3. Yellow dotted line separates MZ and CZ in (F′, G′). PSCs are stained with anti-Antp (nuclear red). DNA is shown in blue. Scale bars are 10 μm.