Figure 6 .

nopo affects lymph gland growth and hemocyte differentiation. Primary lobes are outlined with white dotted line. Crystal cell (anti-PPO2, pink) and plasmatocyte maturation (P1, membrane, red) are increased in middle third-instar (B) and late third-instar (C) nopo^null lymph glands compared with that in wild type (A). Secondary lobes (SL) do not express differentiation markers. Confocal cross-sections through the inner cell layers (A, B) show drastic decrease in mutant MZ size (yellow dotted line). Staining with phalloidin (green) shows cell shape. Staining for Lozenge (Lz), an early crystal cell marker (D–E′, green/white), shows greater than 5 times increase in number of crystal cells in nopo^null lymph gland lobes (E, E′) compared with that in wild type (D, D′).