. 2012 Mar 1;2(3):393–405. doi: 10.1534/g3.111.001693

Table 1 . Summary of the screen for genetic modifiers of Zfrp8/+ lymph gland phenotype.

Deficiency Modifying Zfrp8/+ Lymph Gland Overgrowth	Deleted Segment, Cytology	Numbers of Lymph Glands with Phenotype/ Total Analyzed	Comment on Mapping	Modifying Alleles and RNAi Lines
Suppressing
Df(2L)BSC31	23E5–23F5	7/13	Smaller deficiencies tested, no modifiers found.	n/i
Df(2L)BSC17	30C3–30F1	14/14	Mapped to 30C1 and 30C5. Two dominant suppressors identified.	pelo¹, hoip^k07104
Df(2R)cn9	42E–44C1	20/27	Not mapped.	n/i
Df(2R)BSC29	45D3–45F6	7/12	Mapped to 45F5–46A1.	CG30338 {v 29189}
Df(2R)en-A	47D3–48B2	6/11	Not mapped.	n/i
Df2R)BSC11	50E6–51E4	10/10	Mapped to 51A5—51C1.	n/i
Df(2R)PC4	55A1–55F2	9/10	Mapped to 55B11–55C9.	nopo {v104477} Idgf5 {v100977}
Df(2R)BSC22	56D7–56F12	10/19	Not mapped.	n/i
Df(2R)vir130	59B1–59E1	17/17	Smaller deficiencies tested, no modifiers found.	n/i
Df(2R)or-Br6	59D5–60B8	13/24	Weak modifier mapped to 59D9.	CG30415^EY04039
Df(2R)M60E	60E6–60E11	20/21	Not mapped.
Df(3L)BSC23	62E8–63B6	8/12	Not mapped.
Df(3L)AC1	67A2–67D13	10/14	Two dominant suppressors identified.	shc^KG06847, vs.g ^EY05552, MTF-1 {v107124}
Df(3L)Pc-2q	78C5–79A1	9/15	Not mapped.	n/i
Df(3R)mbc-30	95A5–95C11	9/10	Mapped to 95B1–95D1.	n/i

Modifying			Phenotype of trans-heterozygous lymph glands. Comment on mapping.
Df(2L)net-PMF	21A1–21B8	9/12	Small CZ. Both enhancer and suppressor are found.	l(2)gl⁴, smo³
Df(2R)Egfr5	57D2–58D1	9/9	Small lymph gland with changed morphology. Not mapped.	n/i
Df(3L)emc-E12	61A–61D3	21/28	Lymph glands are enlarged and disintegrated. Enhancing region mapped to 61A5–61B1 and one suppressing gene mapped to 61B1.	Pk61C^EP3091, Pk61C⁵,
Df(3L)W10	75A6–75C2	lethal	Smaller deficiencies do not show lethality. Enhancing region is mapped to 74D1–75B11 Enhancer and suppressors found.	Eip75B^A81, Adgf-A^d11616, mus304^D3, n/i
Df(3L)BSC249	79B2–79D1	11/11	Disintegrated primary lobes. Not mapped.	n/i
Df(3R)ME15	81F3–82F7	14/14	Disintegrated primary lobes. Not mapped.	n/i

Zfrp8-independent phenotypes			Lymph gland phenotype of deficiency/+	Comment
Df(2L)ast2	21D1–22B3	15/15	Lymph gland disintegration mapped to 21D4-21E2.	Ush, lwr are known to cause hematopoietic phenotype
Df(2L)c144	22F4–23C3	15/17	Increased size and differentiation.
Df(2L)spd^j2	27B2–27F2	10/10	Decreased size and small CZ.
Df(2R)cn9	42E–44C1	27/27	Decreased size, increased differentiation.
Df(2L)TW161	38A6–40B1	8/11	Decreased size, increased differentiation.
Df(3L)XG5	71C2–72C1	19/19	Enlarged cortex.
Df(3R)BSC38	85F1–86C8	6/10	Enlarged.
Df(3R)T-32	86D9–87C4	13/17	Enlarged disintegrated.
Df(3R)ea	88E7–89A1	18/18	Enlarged disintegrated.
Df(3R)crb87-5	95F8–96A20	20/20	Primary lobe enlargement.

Columns 1 and 2 show the deficiencies that modify the Zfrp8^null/+ phenotype in more than 50% of the lymph glands and their cytological location. Column 3 shows the number of lymph glands with modified phenotypes relative to the total number analyzed. Column 4 shows the smallest cytological interval to which the modifying effect was mapped. Column 5 lists the genes that were found to have modifying effect. n/i, modifying genes were not identified.