Table 1.
Differential leukemia initiating cell frequency in the G374 line by different assays.
Limiting dilution assay was performed in NS and NSG mice transplanted with variable numbers of G374 cells IV or IF. The proportion of positive mice in each group was determined by FACS analysis of GFP+ leukemia cells in blood, BM and spleen, and splenomegaly in mice that showed signs of illness or died. LIC frequencies and 95% CI (Confidence Interval) were calculated by Poisson statistics and the method of maximum likelihood using L-Calc software (StemCell Technologies).
| Mouse Strain | Cell Dose | No of mice with leukemia/ no of mice tested |
Frequency (95% CI) |
|---|---|---|---|
|
NS
IV |
100,000 | 2/2 | 1 in 43,267 (1 in 9,332-1 in 200,617) |
| 10,000 | 0/2 | ||
| 1,000 | 0/2 | ||
| 100 | 0/1 | ||
|
| |||
|
NSG
IV |
1,000,000 | 2/2 | 1 in 4, 326 (1 in 933-1 in 20,061) |
| 100,000 | 2/2 | ||
| 10,000 | 2/2 | ||
| 1,000 | 0/2 | ||
| 100 | 0/2 | ||
|
| |||
|
NS
IF |
2,500,000 | 1/1 | 1 in 1,609 (1 in 281-1 in 9,211) |
| 500,000 | 2/2 | ||
| 100,000 | 3/3 | ||
| 10,000 | 3/3 | ||
| 1,000 | 1/2 | ||
| 100 | 0/2 | ||
| 10 | 0/2 | ||
|
| |||
|
NSG
IF |
500,000 | 1/1 | 1 in 4, 341 (1 in 939-1 in 20,080) |
| 100,000 | 2/2 | ||
| 10,000 | 2/2 | ||
| 1,000 | 0/2 | ||
| 100 | 0/2 | ||
| 10 | 0/2 | ||