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. 2012 Mar 1;7(3):e32624. doi: 10.1371/journal.pone.0032624

Figure 3. The MEK inhibitor, PD98059, abolishes the LMP1-increased 3′Eκ enhancer activity.

Figure 3

HNE2 and HNE2-LMP1 cells were co-transfected with pβ-3′Eκwt, pGL3-β, or pGL3-Basic and the internal control pRL-SV40 plasmids. Cells were incubated for 24 hr and then treated with PD98059 (50 µM) or DMSO (0.1%) for an additional 12 hr after which activity of firefly and Renilla luciferase was monitored as described in “Materials and Methods”. Values for firefly luciferase activity were normalized to those obtained for Renilla luciferase activity. Values obtained for pβ-3′Eκwt- or pGL3-β-transfected cells were divided by the corresponding values obtained for pGL3-Basic-transfected cells. Data are shown as means ± S.D. of three independent experiments performed in triplicate. Statistical significance: *p<0.05.