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. 2012 Mar 1;7(3):e32052. doi: 10.1371/journal.pone.0032052

Figure 4. CBB staining and immunoblot analysis of four kinds of monoclonal antibodies (N1-8, N1-216, N3-2, and N3-42).

Figure 4

N1-8 did not recognize aa residues 25–254, but recognized aa residues 25–271 of N1. Therefore, N1-8 was considered to recognize an epitope located among aa residues 255–271 of RB1CC1. Similarly, N1-216, N3-2, and N3-42 recognized epitopes located among aa residues 235–254, 715–734, and 715–734, respectively. The recombinant mutant proteins (∼27–56 kDa) of N1 and N3 were confirmed by anti-GST immunoblots. Coomassie Brilliant Blue (CBB) staining of each protein band in polyacrylamide gel electrophoresis is shown in the lowest row. Arrows and arrowheads indicate 47 and 39 kDa, respectively.