Table 1. Analysis of spiked carcinoma cells in human whole blood on a cell microarray chip.
Sample* | number of Leukocytes | spike rate | number of cells** | number of leukocytes | number of carcinoma cells | estimated spiked rate |
(/10 ml) | (%) | (/microchamber) | (/chip) | (/chip) | (%) | |
1 | 25,600,000 | 0.000195 | 89±3.1 | 1,860,000 | 4 | 0.000215 |
2 | 26,200,000 | 0.000191 | 90±2.7 | 1,880,000 | 4 | 0.000213 |
3 | 16,800,000 | 0.000298 | 87±2.2 | 1,820,000 | 5 | 0.000275 |
4 | 27,800,000 | 0.000180 | 89±4.2 | 1,860,000 | 3 | 0.000161 |
5 | 25,400,000 | 0.00197 | 89±5.2 | 1,860,000 | 38 | 0.00204 |
6 | 26,200,000 | 0.00191 | 87±2.3 | 1,820,000 | 37 | 0.00203 |
7 | 27,600,000 | 0.00181 | 89±3.7 | 1,860,000 | 34 | 0.00183 |
*Samples no. 1 to 4 were spiked with 50 carcinoma cells; and 5 to 7, were spiked with 500 carcinoma cells,
**Ten microchambers were randomly selected on a cell microarray chip, and the number of carcinoma cells in each of the chambers was counted.