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(A) Effects of RGMa peptide 4 on RGMa-induced growth cone collapse. Cortical neurons were treated with RGMa and/or RGMa peptides for 30 min. The results were quantified from 3 independent experiments, and the percentage of collapsed growth cones is shown (**P<0.01). Pep4, peptide 4; PepA, Peptide A; PepB, peptide B. (B) Representative images of growth cones. Red: Phalloidin, Green: Tuj1. Scale bar: 10 µm.
