Figure 2. Unimpaired protective capacity of MVA immunization in mice lacking selected key adaptors of innate signaling pathways, or natural killer (NK) cells.

(A) C57BL/6 mice (wt), (B) MyD88/Trif^−/−, (C) IPS^−/− knock-out mice, or (D) NK cell depleted C57/BL/6 mice were challenged with 3×LD₅₀ ECTV two days after MVA immunization (○), with mock-challenged (▪), and mock-vaccinated (▴) animals as controls. In all experiments weight loss of individual mice was monitored daily (n = 2 to 6 per group). +indicate the individual time of death. Error bars indicate SEMs, and the numbers of surviving/total animals are given in parentheses. Statistical significance of differences between groups is indicated by * for p-value<0.05, ** for p-value<0.01 and *** for p-value<0.001.