Fig. 6.

Lack of BTN1 leads to alterations in the phospholipids profile of yeast cells. Yeast cells were inoculated at initial A₆₆₀ 0.1 in SC medium with or without 5 mM ethanolamine and grown for 24 hours, then the indicated cell fractions were isolated and lipid extracts were obtained as described in the Methods. The plots represent the mean and standard errors of the results obtained from five identical experiments. Differences in lipid content are statistically significant with P<0.005 as determined by standard t-test. TF, total fraction; Mito, mitochondrial fraction; MAM, mitochondria-associated membrane fraction; VAC, vacuolar fraction. Differences in lipid content are statistically significant with P<0.005 as determined by standard t-test. Symbols denote statistically significant differences to the same membrane fraction of wild-type (*), psd1-Δ (+) or btn1-Δ psd2-Δ (#) strains grown in the absence of ethanolamine.