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Yeast cells lacking either BTN1 or genes involved in the Kennedy pathway for PtdEtn synthesis show sensitivity to high concentrations of extracellular ethanolamine. Equal number of cells from freshly grown yeast cultures were deposited as undiluted, 1:10, 1:100 or 1:1000 dilutions (left to right) on SC-glucose plates with or without ethanolamine (Etn) and incubated at 30°C for 48 hours.
