Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Nov 3;5(2):220–230. doi: 10.1242/dmm.008409

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

PMC Copyright notice

Fig. 3. — The expression of cardiac markers in iPSC-derived cardiomyocytes and the electrical properties of the cells. (A) Immunocytochemical staining of different cardiac markers: troponin T and α-actinin are shown in red; green indicates connexin-43 and blue represents DAPI-staining for nuclei. The expression was similar in LQT2-specific and control cells, and there were no line-specific differences in the expression of cardiac proteins. (B) The expression of a larger repertoire of cardiac markers was also studied, with RT-PCR showing that the iPSC-derived cardiac cells manifest cardiac properties. TNTT2, MLC2V, MLC2A, Cx-43, MYH7, GATA4 and HERG were present in the cells at the mRNA level. (C) Electrical properties of the cells were studied with MEA, which revealed the differences between LQT2-specific and control cells. FPD was significantly longer in LQT2-specific cardiomyocytes than in control cardiac cells. However, all lines evince the typical electrical properties of cardiomyocytes. (D) LQT2-specific cardiomyocytes showed increased chronotrophy when challenged with isoprenaline, a β-adrenergic agonist.