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. 2011 Nov 1;5(2):248–258. doi: 10.1242/dmm.008383

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© 2012. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 5. — The free amino acid profile of que mutants shows elevated levels of BCAAs at 96 hpf. The amino acids are referred to by their three-letter code, except for GABA. Each experiment contained a homogenate of 50 larvae. The error bars indicate standard error. (A) The free amino acid profile for wild-type larvae (n=3). (B) The free amino acid profile of que mutant larvae reveals a dramatic accumulation of BCAAs: isoleucine, leucine and valine. Other amino acid levels were reduced. * Significant difference from wild-type at P<0.05; ** significant difference from wild-type at P<0.01 (n=3). (C) The free amino acid profile of beo, a zebrafish mutant that demonstrates abnormal behavior owing to a CNS defect, indicates that abnormal behavior alone does not markedly alter free amino acid levels (n=1).