Figure 3. Increased N2 expression can compensate the absence of N1 on L. major stimulated CD4⁺ T cells.

(A–C) Three weeks after L. major infection, dLN cells from the indicated mouse strains were isolated and restimulated for 16 h with UV-treated L. major. Notch1 (A), Notch2 (B), Notch3 and Notch4 (C) expression by CD4⁺ T cells was assessed by FACS. CD11c⁺CD8α⁺ splenic dendritic cells and CD4⁻CD8⁻CD25⁺ thymocytes were stained as positive controls for Notch3 and Notch4 respectively. Representative flow cytometry plots are shown. Numbers in plots represent mean fluorescence intensity MFI ± SEM of ≥3 mice per group. Data are representative of 3 independent experiments.