Figure 4. Coexpression of Aurora A enhances LANA-mediated repression of p53 transcriptional activity which depends on its kinase activity.

Ten million of Saos-2 (A), MEF (B), or 293 (C) cells were co-transfected 3 µg p53 reporter plasmid with construct expressing LANA, Aurora A (WT or KR) or both. At 24 hr post-transfection, cells were harvested and lysed for luciferase assays. The expression levels of each target proteins were detected by westernblotting and shown at the bottom panels. β-actin blot was used as loading control.