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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Endocrine. 2011 Nov 1;41(2):227–235. doi: 10.1007/s12020-011-9548-0

Figure 3. Leptin gene expression in subcutaneous and visceral fat.

Figure 3

At the conclusion of the 10 day diet experiment, subcutaneous and visceral fat were collected and leptin gene expression assessed by real-time RT-PCR. Data were normalized to 18s. All expression is shown as fold-change relative to that in visceral fat of ad libitum fed, non-pregnant mice. Error bars represent 2ΔΔCtCt ±SEM. Comparisons among treatment groups were made by three-way ANOVA and Holm-Sidak post-tests. Diet (restricted vs ad libitum) and reproductive state (pregnant vs non-pregnant) but not fat depot had significant overall effects on LEP expression.