Table 3.
All primers and probes for each SNP in our research
| SNPs | Amplification primers | TaqMan probes or sequencing primers | ||
|---|---|---|---|---|
| Forward | Reverse | |||
| G1 | CTTCCTCTCAGTCTGACCATTGTTC | GCGGGAGCACTCTGACCTT | FAM-CATTCCATGGTACTCG-MGB | HEX-CATTCCGTGGTACTC-MGB |
| G2 | CGGGAGCACTCTGACCTTACC | TCCTCTCAGTCTGACCATTGTTCTT | FAM-CACCTGCACCCGACAGTGATGAGT-TRAMA | HEX-TCCACCTGCACCTGACAGTGATGAG-TRAMA |
| G3 | GTAGGAGTGAGATTCGCTGAAGTTC | CCCCCTACTGGCCATTTGA | FAM-CCTCGCTCTGCCCTCATGGG-TAMRA | HEX-CCTCGCTCTACCCTCATGGGGC-TAMRA |
| G4 | CCGCCTGCACGAGACTGT | CTTCCGGTCCCAAGCATTC | FAM-CAACATCTGGACGGCAGGTAAGTTCC-TAMRA | HEX-CAACATCTGGACAGCAGGTAAGTTCCG-TAMRA |
| G5 | CCCGCGAGGGAAGGTT | GGAGAAAAAGGGCCAGCAA | FAM-CCTCCGCCCCGCTCCTCT-TAMRA | HEX-CCTCCGCCCAGCTCCTCTCTG-TAMRA |
| G6–G9 | TGCTCCTGACACACCCA | AGAGGCACGAGAGGAAC | TGCTCCTGACACACCCA | |
| G10, G11 | AACAAACCCAACCCAAACCA | CCTGCCTCCTACATTCA | TACACGGCACTGCTGAA | |
| G12–G14 | AACAAACCCAACCCAAACCA | CCTGCCTCCTACATTCA | CCGTGTAGACAGCCTTGG | |
| G15 | GCAAGGTGCTGGTGGGTCT | ATTGCTGCCATCCTGGAAGA | FAM-CTGGACGTCGCGGTGTGTCCT-TAMRA | HEX-TGGACGTCGCAGTGTGTCCTCTG-TAMRA |
| G16 | TTCCAGGATGGCAGCAATG | CGGTCCCAGTCCACATGAGT | FAM-TGGAGCGCCCGCCGTC -TAMRA | HEX TCTGTGGAGCTCCCGCCGTC -TAMRA |