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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Genes Immun. 2011 Sep 8;13(2):129–138. doi: 10.1038/gene.2011.62

Figure 3.

Figure 3

Expression of endogenous BANK1 in lymphoblastoid cell lines.

(A) mRNA expression levels of the full-length BANK1 isoform in 16 lymphoblastoid cell lines homozygous for the GG genotype, 11 heterozygous and 6 homozygous lines for the AA genotype of rs10516487. GG vs AA, p=0.013. Expression was normalized to the levels of the 18S rRNA. The mean values +/− SD are shown.

(B) Protein expression in individual lymphoblastoid cell lines as determined by Western blotting with anti-human anti-BANK1 antibody (1:300) detecting the full-length protein. The blots were re-probed with anti-β-Tubulin (1:500) to ensure equal loading amounts and transfer.

(C) Normalized amounts of BANK1 protein to the arbitrarily chosen reference cell line AA3. The amount of BANK1 protein in this line was defined as 100.

(D) Averaged amounts of BANK1 protein measured on the panel C for four homozygous for GG and four homozygous for AA cell lines. Error bars represent +/− SD. GG vs AA, p=0.01.