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. Author manuscript; available in PMC: 2013 Jan 19.
Published in final edited form as: Oncogene. 2011 Nov 21;31(29):3397–3408. doi: 10.1038/onc.2011.515

Figure 5. MAPK activation by PAK1 expression is required for anchorage-independent growth.

Figure 5

A. Phosphorylation status of the MAPK pathway downstream effectors ERK1/2T202/Y204 in HMLE expressing a control vector, PAK1 or PAK1K299R. Total ERK1/2 levels are also shown. B. Anchorage-independent colony formation by HMLE-PAK1 and SUM52 expressing RAF1- or lacZ-specific shRNAs. Corresponding RAF1 protein levels are shown. C. Anchorage-independent growth of HMLE-PAK1, HMLER, SUM52 and EFM19 treated with U0126 at the indicated doses. Representative phospho-ERK1/2T202/Y204 levels are shown. D. Anchorage-independent colony formation by HMLER cells expressing PAK1- or lacZ-specific shRNAs. PAK1 protein levels are shown. Error bars = 1 SD. ***p-value <0.0001. **p-value <0.001.