Fig. 9.

Effect of nicotinamide on PARP-1 activity in brain and heart from asphyxia-exposed and control rat neonates. PARP-1 activity was measured by a PARP Universal Colorimetric Assay (R&D Systems, Minneapolis, MN; catalogue #4677-096-K) in heart (a, b) and brain (c, d) and tissue dissected from asphyxia-exposed (AS, n = 5; AN, n = 5) and control (CS, n = 5; CN, n = 5) animals, treated with saline (1 ml/kg, i.p.) (AS, CS), or nicotinamide (0.8 mmol/kg, i.p.) (CN, AN) 1 h after delivery. The animals were dissected 1 h or 8 h after a single administration of saline or nicotinamide. Open and grey columns PARP-1 activity in CS and AS pups, respectively. Dashed columns PARP-1 activity in CN and AN pups, respectively. * P < 0.05 (Allende et al. in preparation)