Table 2.
Motility inhibitory and zoosporicidal activity of DAPG and its derivatives against the downy mildew pathogen Plasmopara viticola
| Compound | Dose (μg/ml) | Motility inhibitory and zoosporicidal activity (% ± SE)a | |||||||
|---|---|---|---|---|---|---|---|---|---|
| 15 min | 30 min | 45 min | 60 min | ||||||
| Inhibited motility | Lysed | Inhibited motility | Lysed | Inhibited motility | Lysed | Inhibited motility | Lysed | ||
| PG | 1 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 |
| 10 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 7 ± 1 | 0 ± 0 | 11 ± 3 | 3 ± 1 | |
| 100 | 0 ± 0 | 0 ± 0 | 21 ± 0 | 0 ± 0 | 37 ± 9 | 9 ± 2 | 42 ± 5 | 12 ± 4 | |
| MAPG | 1 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 |
| 10 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 18 ± 3 | 6 ± 1 | 47 ± 5 | 18** ± 0 | |
| 50 | 30 ± 6 | 9 ± 2 | 72 ± 5 | 39 ± 3 | 76 ± 10 | 42 ± 4 | 82 ± 7 | 58 ± 7 | |
| 100 | 100 ± 0 | 100 ± 0 | nt | nt | nt | nt | nt | nt | |
| DAPG | 1 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 |
| 10 | 0 ± 0 | 0 ± 0 | 13 ± 3 | 0 ± 0 | 21 ± 4 | 5 ± 1 | 29 ± 5 | 17* ± 4 | |
| 15 | 72 ± 9 | 43 ± 5 | 93 ± 10 | 76 ± 7 | 95 ± 4 | 82 ± 6 | 98 ± 3 | 89 ± 8 | |
| 20 | 92 ± 4 | 55 ± 6 | 100 ± 0 | 88 ± 11 | 100 ± 0 | 98 ± 1 | 100 ± 0 | 100 ± 0 | |
| 25 | 100 ± 0 | 98 ± 3 | 100 ± 0 | 100 ± 0 | nt | nt | nt | nt | |
| TAPG | 1* | 0 ± 0 | 0 ± 0 | 10 ± 3 | 0 ± 0 | 15 ± 2 | 5 ± 1 | 33 ± 6 | 18 ± 3 |
| 5* | 20 ± 3 | 0 ± 0 | 41 ± 5 | 31 ± 6 | 68 ± 8 | 42 ± 8 | 73 ± 10 | 51 ± 7 | |
| 10* | 39 ± 6 | 8 ± 2 | 61 ± 8 | 44 ± 5 | 81 ± 9 | 61 ± 6 | 98 ± 3 | 76 ± 10 | |
| 15 | 74 ± 7 | 21 ± 6 | 89 ± 9 | 65 ± 9 | 100 ± 0 | 90 ± 7 | 100 ± 0 | 100 ± 0 | |
| 25 | 91 ± 3 | 80 ± 7 | 100 ± 0 | 100 ± 0 | nt | 100 ± 0 | nt | nt | |
| DPPG | 1* | 0 ± 0 | 0 ± 0 | 12 ± 3 | 0 ± 0 | 21 ± 5 | 0 ± 0 | 39 ± 4 | 18 ± 3 |
| 2 | 80 ± 5 | 68 ± 8 | 90 ± 7 | 72 ± 8 | 97 ± 4 | 82 ± 5 | 99 ± 1 | 86 ± 7 | |
| 3 | 100 ± 0 | 85 ± 11 | 100 ± 0 | 99 ± 2 | 100 ± 0 | 100 ± 0 | nt | nt | |
| 4 | 100 ± 0 | 100 ± 0 | nt | nt | nt | nt | nt | nt | |
nt not tested, PG phloroglucinol, MAPG monoacetylphloroglucinol, DAPG diacetylphloroglucinol, TAPG triacetylphloroglucinol, DPPG dipropylphloroglucinol
* Zoospores were moving very slowly with tight circle or jerky movement
** Only larger zoospores stopped and lysed
aData presented here are average value ± SE of at least three replications in each dose of compound