Fig. 5.

Total inflammation scores 3 weeks after AOM application. WT and GPx2-KO mice on different selenium diet were exposed or not to AOM/DSS with and without SFN as indicated and inflammation scored 1 week after DSS treatment (see Figure 1) by clinical, macroscopical and histological signs (see Materials and methods). Only AOM/DSS-treated groups are shown. Gray background, WT; white background, GPx2-KO mice. Striped boxes indicate SFN4. Data are box plots with median and whiskers from minimum to maximum (n = 4). Significance of different treatments is marked by the same symbols used in Figure 3. * Difference between genotypes in the same selenium group. ^# SFN effect versus the respective untreated group and ° selenium effect as indicated above horizontal lines or versus the respective Se-poor group. One symbol, P < 0.05; two symbols, P < 0.01; three symbols, P < 0.001 estimated by two-way analysis of variance and Bonferroni’s post tests. WT +Se versus WT ++Se is significant (P < 0.05) when analyzed with Mann–Whitney U test indicated by ⁽°⁾. ⁽*⁾ indicates the trend with P = 0.102 versus WT in the same Se group, estimated with Student’s t-test.