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Neutrophil numbers and mediators of innate immunity after final allergen challenge. Cytospin preparations from cells collected in the BAL fluid were made and 300 cell differential counts performed to determine the absolute numbers of neutrophils (a). Concentrations of chemokines CXCL1(b), CXCL2 (c) and TNFα (d) in the BAL fluid were measured by standard ELISA. Data are presented as mean ± SEM (n = 4 mice per group per experiment; combination of 3 separate experiments).
