Fig. 4.

Eosinophil recruitment and production of eosinophil-specific chemokines in response to allergen challenge. Cytospin preparations from cells isolated from the BAL fluid were made and 300 cell differential counts performed to determine the absolute numbers of eosinophils at 24 h after final challenge (a). Concentrations of the chemokines CCL11 (b) and CCL24 (c) in the BAL fluid were measured by standard ELISA. d Eosinophil peroxidase activity was measured in the lung tissue after BAL at 24 h after final challenge. Concentrations of CCL11 (e) and CCL24 (f) in the lung homogenate (LH) were assayed by standard ELISA. Data are presented as the mean ± SEM (n = 4 mice per group, per experiment; combination of 3 separate experiments). * p < 0.05, *** p < 0.0001 compared to nontolerant group.