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. 2009 Jun 3;9(6):4272–4285. doi: 10.3390/s90604272

Figure 1.

Figure 1.

Synthesis of cma and corS mRNAs at 18 °C, 28 °C and after a temperature downshift from 28 to 18 °C, as determined by RNA spot blot analysis. (A) RNA spot blot data for cma and corS mRNAs at 18 and 28 °C. Samples were withdrawn from cultures at OD600 of 1·0. mRNA levels at 18°C were defined as 100%. (B) RNA spot blot analysis for cma and corS mRNAs after a temperature downshift from 28 to 18°C. Bacteria were grown in HSC medium at 28 °C to an OD600 of 0·5 and subsequently shifted to 18 °C. Total RNA was isolated from samples withdrawn from PG4180-cultures at different time points after the downshift in temperature and subjected to RNA spot blot analysis. Quantities represent averages of two experiments with six replicates. The relative mRNA level is related to the level of the respective mRNA synthesized at 18 °C (A), which was defined as 100%.