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. 2009 Jun 3;9(6):4272–4285. doi: 10.3390/s90604272

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© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 3. — Stability of the cma transcript as a function of temperature. Transcription was blocked by addition of rifampicin (0 min) at 50 μg mL^-1 to the 18 °C-culture and the culture shifted from 18 to 28 °C at an OD₆₀₀ of 1·0. Total RNA was isolated from aliquots harvested at different time points after rifampicin addition and subjected to RNA spot blot analysis. The amount of detected mRNA at zero minutes was considered as 100%. The percentage of transcript remaining after rifampicin addition was plotted as a function of time.