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(A). Schematic representation of Gαt chimeras. The switch III region (S3, Gαt1-228-236) (black) is identical in Gαt1 and Gαt2. (B). Coomassie blue-stained SDS-gel showing purified Gαt chimeras. Samples represent equal fractions of the preparations from 0.5 liter bacterial cultures.